Effect of Plasma-Activated Water with Varying RONS Concentrations on Barley Seed Germination Parameters

Abstract

The objective of this study will be to reveal the correlation between the concentration of reactive oxygen nitrogen species (RONS) and its impact of seed germination and initial development of a plant. The activation was done by using plasma jet system, with AC voltage sine wave from 0 -12 kVA, frequency 20 kHz and total power of 60 W. The argon gas at flow rate of 2.5 L/m. 60 cm of distilled water was treated with plasma directly. Each 20 cc was treated at different times. 240 seeds of barley were taken and divided equally into four groups. Three groups were treated with water active by plasma WAP and one group was treated with distilled water. After 24 hours the seeds were planted in germination plates. The plates were put in dry room with a normal temperature. The seeds were monitored for four days after soaking process the monitoring focused on germination percentage GP and the germination rate GR. The results were as follows: S1, soaked with PAW, the total RONS concentration of 201ppm GP 93% and GR 53, S2 soaked with PAW the total RONS concentration of 132ppm GP 88% and GR 48.6, S3, soaked with PAW the total RONS concentration of RONS 77 ppm GP 91% and GR 50.6 . The control group GP 85%, and GR 43.3. The statistical calculation indicated that PAW treatment significantly influenced barley seed germination. These findings conclude that WAP positively affects seed germination rates and initial seedling development.