AP01038 Ag2Se Nanoparticles Synthesis by Co-Precipitation Method: Characterization and Antimicrobial Evaluation

Abstract

Silver selenide (Ag₂Se) nanoparticles possess promising optoelectronic properties, making them attractive for applications in biomedical fields such as bioimaging and biosensing. In this study, Ag₂Se NPs were successfully synthesized via the chemical co-precipitation method. Field emission scanning electron microscopy (FE-SEM) images revealed that the nanoparticles exhibited a tightly packed lamellar-like morphology with particle sizes 48nm. X-ray diffraction (XRD) analysis confirmed the formation of an orthorhombic β-phase structure of Ag₂Se. Energy-dispersive X-ray spectroscopy (EDX) confirmed the elemental composition, showing an atomic ratio of Ag to Se close to the ideal 2:1 stoichiometry. UV–visible spectroscopy showed a distinct absorption peak at 250 nm. The optical dispersion behavior was analyzed using the Wemple–DiDomenico single effective oscillator model, through which the refractive index dispersion, average oscillator energy (Eₒ), and dispersion energy (Ed) were determined. Additionally, the first-order (M-1) and third-order (M-3) spectral moments were calculated based on the extracted Eₒ and Ed values to further interpret the optical transitions. Beyond structural and optical characterization, the antibacterial activity of the synthesized Ag₂Se NPs was also evaluated. The results demonstrated notable antibacterial efficacy, indicating the potential of Ag₂Se nanomaterials for applications in medical, and environmental.