AP0991 Synthesis of Calcium Oxide Nanoparticles and Their Synergism Applications

Abstract

In this study, the calcium oxide nanoparticles (CaO NPs) were synthesized through the calcination of chicken eggshells at 900°C and their subsequent synergistic combination with naringin have been explored. A CaO NPs/naringin composite was synthesized by combining colloidal CaO NPs in a solution of naringin.  Confirmation of the cubic crystalline nature and polydisperse size distribution of the particles was done through X-ray diffraction and transmission electron microscopy (TEM) analysis. The nanoparticles were subjected to an ultrasonic probe, resulting in particles with a mean of 9 nm, along with their synergistic effects, which increased to 19 nm. Antibacterial activity was examined by the agar diffusion assay, with a highest inhibition zone of about 18 ± 0.67 mm for the CaO NPs/naringin mix, and appreciably heightened activity when compared to monotherapies. Statistical analysis upheld the reproducibility of the results (P ≤ 0.001). The results highlight the promise of CaO NPs as powerful nanocarriers for naringin that significantly enhance its antibacterial activity against resistant microbial strains.